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Objective To observe the effects of remote ischemic preconditioning(RIPC) on Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme in thoracoscopic heart operation.Methods One hundred and twenty patients with thoracoscopic heart operation were randomly divided into the thoracoscopic extracorporeal circulation control group (C)and RIPC plus thoracoscopic extracorporeal circulation group(RIPC).The acidity and alkalinity change of arterial blood before and after treatment was abserved in the RIPC group;the changes of myocardial enzymes spectrum,cTnI and oxidation indicators(SOD,MDA) were compared among different time periods,and preoperative and postoperative Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme levels were also compared.Results Compared with the group C,the acidity and alkalinity of artery blood were lower after RIPC(P＜0.05);the levels of CK-MB and cTnI at postoperative 6,24 h in the RIPC group were lower than those in those in the group C(P＜0.05);the SOD activity was higher than that in the group C,while the MDA level was lower than that in the group C(P＜0.05);the postoperative Na+-K+-ATP enzyme and Ca2+-Mg2+-ATP enzyme levels were higher than those in the group C at different degrees.Conclusion RIPC can alleviate myocardial injury in the patients with thoracoscopic heart operation and this effect may be related with activation of corresponding ATP enzyme.
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Objective To investigate the assessment of multi-detector spiral CT on the cardiac function after bioprosthetic valve replacement with mitral valve preservation.Methods Thirty-five patients suitable for undergoing bioprosthetic mitral valve replacement in our hospital from May 2012 to February 2014 were randomly divided into 2 groups:group A(experimental group,intraoperative preservation of mitral valve and subvalvular structure during,n=16) and group B (control group,without intraoperative preservation of subvalvular structure of mitral valve,n=19).MDCT before operation and on postoperative 10 d was performed for evaluating the left ventricular function.Results In the comparison of preoperative MDCT,the end diastolic velocity(EDV),end systolic velocity(ESV),ejection fraction(EF) and stroke volume(SV) had no statistical difference between the group A and B.In MDCT results on postoperative 10 d,EDV,ESV and EF in the group A were 113.42±9.22)mL,(38.63±3.40)mL and (51.63±4.71) ％,which in the group B were(113.42 ± 9.22) mL,(38.63 ± 3.40) mL and (51.63 ± 4.71) ％ respectively,the difference was statistically significant(P＜0.05),but SV had no statistical difference between the two groups(P＞0.05).Conclusion The bioprosthetic valve replacement with mitral valve preservation is safe and feasible in technology,moreover effectively protects the postoperative left ventricular function.
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Objective To investigate the protective mechanism of ischemic preconditioning for myocardial ischemic reperfusion injury (MI/RI) rats. Methods Eighteen male Sprague–Dawley (SD) rats were randomly separated into Sham operation,ischemic reperfusion (IR) and ischemic preconditioning (IP) groups (6 rats in each group). Peripheral blood and cardiac muscle were collected after the establishment of the above 3 animal models. ELISA was employed to determine proinflammatory cytokines TNF-? and IL-1? production in sera of these animals. RT-PCR and Western blot analysis was used to assay the transcriptional level and protein level of Toll-like receptor 4 (TLR4) in cardiac muscle tissue with pathological change,respectively. Results Compared with IR group,ischemic preconditioning effectively decreased the expression levels of TNF-? and IL-1? in sera of rats in IP group (P